FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences892543
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACTT9050.025.06611
TTGGGAG23900.024.0080536
GCGCAAG1802.6687337E-422.2400191
CTAGACG1000.00249589122.126802130-134
AAAGTGC18150.022.0562174
GCTGGGA34900.021.7939439
CTACTTG9400.021.2936342
AAGTGCT18550.021.2209345
CTACTCG5100.020.9317822
CGGAGGT6700.020.9122567
AGTAGCT16550.020.5602595
GTAGCTG17850.020.184226
GGAGGCT33250.019.865529
CCAAAGT20250.019.7689062
AGGCGGA10500.019.6983034
TACTCGG6900.019.3391463
GCACTTT16700.018.77751
GGGAGGC44650.018.3797688
GGCGGAG11300.018.3037345
TACTTGG12050.018.2718833
Produced by FastQC (version 0.11.5)