FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences892543
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAA800.00305615733.370491
AGACGAT2000.00491253730.282827145
CTACTCG4650.027.2705062
AATAGCG1300.001051162825.669616
CGAGTAG4900.024.5170963
AGTAGCT15750.023.3063745
TTGGGAG23000.023.2142526
CGGAGGT6250.022.4249697
GTAGCTG16850.022.1809796
TACTCGG5850.021.676563
CTACTTG7700.021.6691472
GAGTAGC10800.021.6290234
AAGCCGC1903.8377996E-421.07614
GCACTTT16250.020.9464021
TTTGGGA18850.020.889755
CTACACG1600.003516159720.8565562
CCGAGTA6150.020.6191652
GCTGGGA35600.019.8723149
TAGCTGG20350.019.677937
AAAGTGC17700.019.2304534
Produced by FastQC (version 0.11.5)