FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1030806
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGCA2150.005692658529.384268145
GAGACTT8900.028.941941
TAACTCG950.006566766628.5410845
TGATCAA9050.026.9642269
GACTTGT9650.025.2876933
CTTGTGA10550.024.4154765
ACTTGTG11000.023.416664
AGACTTG11650.022.6919982
CCTATAT2753.7657264E-722.1842041
GTGATCA11200.021.788068
TTGTGAT12100.021.2878726
GCTAACG1600.003213905521.1828331
TACACGG2004.886159E-420.335523
CTACTCG4303.921741E-918.9167632
CTTATAC2157.9516467E-418.9167632
AAAGTGC14450.018.7640324
CTACACG2250.001079162918.076022
TGTGATC14700.017.9837937
CGTCGAG1900.00867844317.8381772
CCAATAT5802.910383E-1117.530621
Produced by FastQC (version 0.11.5)