FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1030806
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACTA800.00282652733.9070632
GGCGACT2001.7162165E-830.5163561
CTACTAG2150.006008069529.061838145
AAAGTGC15900.023.0312124
ACTTACG1903.4463307E-421.4149864
CCCGTAG1903.4463307E-421.4149861
CCCTAGT3558.914867E-921.0128271
CCAAAGT18500.020.8940812
CGTAGGG2305.2594587E-520.6390823
AAGTGCT17550.020.4794795
CAAAGTG20300.019.0414033
GCTACTT7550.018.8622071
CTACTTG6600.018.494762
CCCAAAG23650.018.0646511
CAAGTAG6700.017.206573
AGTAGCT10250.017.2016325
TTGGGAG19550.016.996896
CTATAGT2400.001658354816.9535312
TATAGTC2500.002178742616.275393
GCTATAA2953.5389123E-416.0914881
Produced by FastQC (version 0.11.5)