FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences875854
Sequences flagged as poor quality0
Sequence length35-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG5150.035.5143662
CGAGTAG3400.033.103893
TACTCGG5850.032.4672783
CGCGGGT1454.2874017E-529.1085919
CTACGGT1754.7725207E-628.1383066
GCTACTC13000.026.514941
ACTCGGG7750.024.5075534
GATAGTG2901.6400008E-824.257161
AGTAGCT13200.023.981515
CCTACGG2351.7295224E-623.9474955
CCGAGTA4500.023.4485862
GTAGCTG15100.021.89576
GCCTACG2253.3463053E-521.8853474
GCTGGGA33650.021.3232339
TACGGTG2003.8039245E-421.1037277
TCGGGAG8800.020.7839746
AGGCGGA11550.020.098794
TTGGGAG21450.020.0050896
CTCGGGA8800.019.984595
TAGCTGG17450.019.753257
Produced by FastQC (version 0.11.5)