FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1326862
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGCG1557.868093E-539.934227145
ATACCGG850.00306142233.359965
CTACTCG7300.033.017222
TACTCGG8650.027.8642433
CCCGTAA1500.001703272323.6299691
CGGAGGT9150.022.467847
ACTCGGG11000.021.9114274
TCGGGAG12050.021.7670256
GCCGTAA1700.003523489520.8499741
GCTACTC17600.020.5419641
AAGTGCT23150.020.2105125
GGAGGCT44150.019.5890589
AAAGTGC23400.019.3886934
TTGGGAG32250.019.3436036
GCAACGT1850.00574739219.1594358
CTACTTG12600.019.1290252
CTCGGGA12600.019.1290255
AGTAGCT19400.018.6360055
CCAAAGT27900.018.5482562
CCCAAAG32550.018.5119571
Produced by FastQC (version 0.11.5)