FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066343
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA301.04940525E-4150.88448145
CGACTAA750.004002741560.35379145
GATACGA850.006568867253.25335145
CTATATA2601.9969775E-723.6924742
CTACTCG5850.021.0599782
CTACTTG8000.020.5334762
GCTACTC12900.020.162071
GCTAGTA1750.00510542119.5556931
GCTACTT9150.019.448831
TACAACG1800.006008036419.012483
TACTCGG6250.018.617023
CCTAATT4205.5233613E-817.9260521
AAAGTGC17950.017.5402054
CGAGTAG4006.515638E-717.111233
CCTATAT3304.116477E-516.5927091
TTGGGAG20850.016.413656
TAGCTGG15850.015.9776817
CCAAAGT21150.015.2099852
ACTATAT3155.431848E-415.2099841
CCTAAAT4101.557121E-515.0244971
Produced by FastQC (version 0.11.5)