FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1109257
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGA1501.364353E-633.042212
AGTCGTT1850.00721424927.994812145
GACTTGT8900.023.0712053
TGATCAA9000.022.8148579
CTACTCG4405.456968E-1222.528782
CCGCTAG1600.002495828822.126482
GAGACTT9650.022.0118371
CTACTTG7550.021.5696542
AAAGTGC18200.021.3970364
TCGCGAG2003.6413228E-421.241423
AGACTTG10250.020.7233372
CTTGTGA10050.020.4312175
ACTTGTG11050.019.8637714
CGAGTAG4652.8194336E-1019.794873
GCTACTT8650.019.6452451
AAGTGCT20100.018.6699055
GTGATCA11000.018.6667028
TATGGCG1900.006750241418.6328245
GCCGCTA1900.006750241418.6328241
TTGTGAT10750.018.4421636
Produced by FastQC (version 0.11.5)