FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022787
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACT850.003116296133.2385069
ACCCGTA1000.00690056128.252739
AAAGTGC17950.023.6095224
CTACTCG4850.023.3012222
TGATCAA8250.022.2597279
GAGACTT8400.021.8622321
GACTTGT8150.021.6662033
AGCTCGT3753.9088662E-421.016804145
AAGTGCT20300.020.87645
GCTACTC12300.020.672731
CCAAAGT18450.020.672732
CGATTTG1750.00425595720.1805212
ATCGGGT1750.00425595720.1805215
CAAAGTG22200.020.0441673
ACTTGTG10050.018.9757164
CCCAAAG23750.018.7360231
GCTATAT3051.5966705E-518.5263791
AGACTTG9950.018.4565562
GTCGAGA2400.001262552117.6579571
GCTAATA3202.4132574E-517.6579571
Produced by FastQC (version 0.11.5)