FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1201029
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA600.002967422165.09231145
TCCGTTA952.656733E-454.81458145
CGTCGAG2406.002665E-1132.3528481
CGTATGG900.00413572131.3724583
GTCGAGA2803.583409E-1027.731012
GCGTGTT2106.299688E-726.890681
AAAGTGC21150.024.3637164
ACGTCGA2202.7406952E-522.459831
AAGTGCT22350.022.1081075
CCAAAGT21900.021.9177442
ATATGCG1650.003037557221.3903124
CTACTCG5950.021.354362
CCGACGA1750.0042716920.1680092
CAAAGTG25750.019.4631083
CGGCGAA1850.005890525419.0778477
CCCAAAG27250.018.6507821
GTCGTCG2309.529884E-418.4142681
GCTACTC13500.017.7777271
GAGACTT11150.017.7261431
TGATCAA9600.017.6470079
Produced by FastQC (version 0.11.5)