FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences892699
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGG1806.346706E-541.411125145
CGTCGAG1509.113137E-1041.2143331
GTCGAGA2001.5139449E-830.9107512
CTACTCG3950.027.8240222
CCGATAC1000.00790550127.4762252
TGATCAA7900.025.215529
CGAGTAG3151.9317667E-923.9871773
GACTTGT8350.023.85663
CGAGACT8250.023.313161
GAGACTT9200.023.1457311
CCGTATG1550.002474746722.1582452
AGACTTG10700.019.2590332
GCTATTG2509.0450936E-519.2333551
GCCTATT2901.3142466E-518.949121
GTGATCA11100.018.5650169
CTTGTGA10400.018.4936125
ACTTGTG10850.018.3596884
TACTCGG4959.731593E-1018.0399443
TTGTGAT11250.017.70696
AGTAGCT11350.016.9456885
Produced by FastQC (version 0.11.5)