FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences852865
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACTA400.00733152651.7978862
GATACGA804.9737144E-543.1649061
CTAGCGC750.001882769236.8340532
CGTCGAG1159.9634E-636.033312
GCTAGCG850.00347825432.5006331
TGATCAA7900.032.346369
ATACGAG900.00460116530.6950442
GAGACTT9250.030.6120821
GACTTGT8800.029.8230253
ACTTGTG9850.029.4485454
GTGATCA8850.028.8741518
CTTGTGA9700.028.4799375
ATAATCG1000.007698989527.6255424
AGACTTG10400.026.5630192
GCTAGTC1308.608639E-426.5630191
CATAGCG2850.001158219125.242264145
GTCGAGA1701.4338468E-424.3754753
TTGTGAT11400.023.6271066
GATATAT2803.7398968E-722.1990951
CTACTCG3608.481948E-921.1028422
Produced by FastQC (version 0.11.5)