FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732267
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG553.5567573E-451.670332
ACGTCGA700.001167101640.5981181
CTACTCG3450.030.8898722
TACTCGG4550.026.5449243
CGGAGGT4950.025.8351657
CCTATTA1951.0239046E-525.5039461
ACTCGGG5700.023.6822364
TCGGGAG5550.023.0421756
GCTACTA1852.0991219E-423.0421731
GCTACTC8650.022.9977781
GCTACTT5650.022.634351
AAGTGCT12050.022.4047725
CCCGCGG2900.00323333921.157322145
AAAGTGC11900.020.896094
CGAGTAG2405.0970528E-520.7219563
CTCGGGA6550.020.6089675
GCTATAC1750.00411297620.2990591
GCTGGGA23300.020.1248199
GGAGGCT23100.019.9914979
GCGGAGG7850.019.0061266
Produced by FastQC (version 0.11.5)