FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732267
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG2900.036.746973
CCGAGTA4100.029.4573272
TCGGTAA1000.006705975628.4176562
CTACTCG3403.6379788E-1227.1639392
AAACCGG1359.096801E-426.3126477
AAAGTGC12600.025.9367524
CGGAGGT4400.025.8342367
AAGTGCT12900.024.782845
AGTAGCT8450.024.3820155
GCTACTC8250.024.1119521
GTCACTA3601.7074566E-523.871159145
CCAAAGT15150.022.9779742
TACTCGG4104.9112714E-1122.5261923
CTACTTG5600.021.5669732
GCTACTT6000.021.3132441
ACTCGGG4701.2732926E-1121.1620854
GGAGGTT11950.020.8079098
GCTGGGA25300.020.779719
CCCAAAG17950.020.1852461
TTGGGAG16150.019.7955826
Produced by FastQC (version 0.11.5)