FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences881664
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA200.006644986148.17358145
TTAACGA700.001358315239.365383
ATCGGGT1103.2871886E-431.3133727
TATCGTT900.004658521630.6175168
AGTCGTT1950.00482506630.39458145
ATCGTTT1951.3008168E-524.7295329
GACTTGT7450.020.3431823
CTACTCG2851.1032867E-519.337382
AAGTGCT13700.019.1080115
TGATCAA7250.019.0039779
CCAAAGT14800.018.6187612
TATCGGG1850.00677864418.6187616
AAAGTGC13000.018.547154
ACTTGTG8950.018.4731394
GTCGAGA1900.00790581418.1287921
GTCTACG1950.00918216917.6639528
GAGACTT7950.017.3306711
GCTACTC7600.017.2223531
TAGTAGG2400.001492354117.2223537
GTGATCA8550.016.9202068
Produced by FastQC (version 0.11.5)