FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1223852
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG1900.043.4464231
GTCGAGA2500.035.7708852
GACTTGT12650.030.4525643
TGATCAA13750.028.5166539
GAGACTT14350.027.8036921
CTTGTGA14950.027.6080935
GTGATCA14500.027.516078
CCCGTAA1000.00785092227.516071
ACTTGTG16150.027.26054
CGATCCG1050.00995970626.205784
AGACTTG15750.024.895492
TTGTGAT17550.023.9099736
CGGTTTA1450.001665610923.7207491
CGAGACT10350.021.9330961
TGTGATC20100.020.534387
AAAGTGC20250.020.0425684
CTACTCG5305.456968E-1219.4689182
AGTAGCT14550.019.384175
CGAGTAG4353.774403E-918.9765993
TAGCTGG15550.018.5799837
Produced by FastQC (version 0.11.5)