FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1223852
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG7000.029.491922
CGTGACG950.00610112728.9745182
TACTCGG9000.021.4089493
CGATAGG1650.003520497120.8528733
AAAGTGC21050.020.5953084
GCTACTC14550.020.3369261
CCAAAGT22500.019.5738982
AAGTGCT22750.019.3587995
AGTAGCT14800.019.063475
TAGCTGG17450.018.9289117
CCGTAAT1850.006822827318.5985091
TTTGGCG1850.006822827318.5985094
CAAAGTG24600.018.4624213
CTCGGGA10900.018.3084375
GGTCTAT3803.5982157E-718.1090741
TCGGGAG11450.018.0299956
CCCAAAG29350.017.3501571
CTACTTG9250.017.1106262
GCTACTT11400.016.9018021
ACTCGGG11550.016.6822994
Produced by FastQC (version 0.11.5)