FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1017731
Sequences flagged as poor quality0
Sequence length35-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG700.001252473440.0209435
CTACTCG5700.035.6326832
CGTCGAG800.002413099235.0183261
TACTCGG6850.030.6729853
CGCGGTT950.005597799629.4891159
GCTACTC12600.025.5689351
ACTCGGG7950.024.6669984
ACGGTAA1852.3137053E-422.714592
TCGGGAG9550.021.2676756
GTCGAGA2003.9193282E-421.0109942
CTCGGGA10350.019.6237955
GCTACTT10250.019.1319641
AGTAGCT15100.019.0165755
GCGGGAT1850.006162818518.9288259
GACGGTA1850.006162818518.9288251
TTGGGAG22100.018.380666
AAAGTGC20100.018.1189374
CTACTTG9750.017.9581172
CGGAGGT7100.017.755777
GCTGGGA36750.017.723569
Produced by FastQC (version 0.11.5)