FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1121654
Sequences flagged as poor quality0
Sequence length35-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC850.00323725332.9814573
CTACTCG6950.032.2696272
TACTCGG7750.028.9385683
CGTGACG1000.007167414328.0342352
CGGAGGT8400.026.6992747
CGCTTAG1050.0090936226.6992741
GCTACTC15700.024.1058731
ACTCGGG9900.022.6539294
AAAGTGC21950.022.3507584
TCGGGAG12000.022.1937716
GGAGGCT38400.020.8066629
CTCGGGA12150.020.1892665
AAGTGCT23900.019.0609365
CCAAAGT25050.019.025232
AGTAGCT17000.018.9643365
TTTCGGA1850.006138390418.9420535
CCCAAAG30000.018.6894911
TTGGGAG28150.018.4239226
CAAAGTG28400.018.2617383
GCTGGGA42750.018.1976629
Produced by FastQC (version 0.11.5)