FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1121654
Sequences flagged as poor quality0
Sequence length35-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGCG750.001751001537.383373
CTACTCG6400.026.2851812
GCTACTC15800.025.7306421
CGGAGGT9600.024.094757
TACTCGG7350.023.8414343
GGAGGCT41350.021.3586969
AGTAGCT16200.020.7685385
CGACTAG1700.003761411420.615833
TCGGGAG11650.020.4565646
CTACTCA9750.020.1295072
AAAGTGC22700.019.7621334
CCAAAGT24700.019.5808642
AAGTGCT24450.019.4943965
AGGCGGA14250.019.183574
TTGGGAG28650.018.8384786
CGAGTAG5601.0913936E-1118.775133
GCTGGGA45250.018.743329
ACTCGGG9800.018.596324
CTCGGGA11750.018.4928385
GGCGGAG16350.018.434465
Produced by FastQC (version 0.11.5)