FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences818297
Sequences flagged as poor quality0
Sequence length35-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAC400.006963359752.477045
AACGACG658.7382906E-443.0580836
CTACTCG4300.026.0351222
CGGAGGT6700.022.9750237
CCGAGTA4600.022.8161052
TACTCGG5400.022.0274013
CTACTTG8400.021.657192
AAAGTGC15900.021.5628934
TTGGGAG19600.021.0622126
GCTACTC10000.020.9908161
AAGTGCT16800.020.4077385
CGAGTAG3901.8335413E-819.7349553
AGGCGGA10700.019.6175844
TATATAG3251.381235E-619.3761373
AGTAGCT11950.019.322095
CCCAAAG21300.019.0526961
CCAAAGT18400.019.013422
CCTATTA2300.001010643718.2528841
GCTAAAC2300.001010643718.2528841
CCTACTA2701.4165496E-418.1402111
Produced by FastQC (version 0.11.5)