FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences818297
Sequences flagged as poor quality0
Sequence length35-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATA350.0040990259.9963877
CGAGTAG2851.4551915E-1129.4719093
CTACTCG4800.029.1649112
CCGAGTA3750.027.9983162
CGGTGTA1556.986622E-527.0951443
CTAGCGC1050.00914922726.665062
GCTACTC11950.026.3582441
AGTAGCT12100.024.8745355
GGAGGCT28200.024.82129
TTGGGAG20250.024.1960746
TACTCGG5550.023.9625233
AAAGTGC15200.023.4854284
GCACTTT13300.022.1039311
CCAATTA4208.367351E-1121.6653611
AAGTGCT16250.021.5371635
GTAGCTG14250.021.1215346
CTACTCA7300.021.094622
CCAAAGT17600.020.6805732
GCTGGGA31500.020.665429
GCTACTT9850.020.6078951
Produced by FastQC (version 0.11.5)