FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences939188
Sequences flagged as poor quality0
Sequence length35-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG4350.035.432752
ATATCGC1204.9585855E-429.1917559
TACTCGG5550.026.509273
AAAGTGC17400.024.9639844
AAGTGCT19150.023.7802285
ACTCGGG6800.023.6968384
GCTACTC10450.023.4651411
TTGGGAG22400.023.457666
CGAGTAG3653.3651304E-1023.0334953
CTATTAG2752.7388705E-722.9287992
CCCAAAG22950.022.2849481
CCAAAGT21800.022.175022
GCTGGGA34950.020.6472179
CTACTTG9000.020.2396162
TCGGGAG7300.020.1543086
CAAAGTG23750.019.4693663
GGAGGCT30300.019.4226349
GCACTTT14550.019.2605421
AGTAGCT14650.019.1290685
CCTAGTA2207.4326183E-419.107332
Produced by FastQC (version 0.11.5)