FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences937725
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAACG300.002132976770.747713
TAACGGG553.6320914E-451.4528775
TACGGGT1001.6066912E-435.3738565
CTACTCG4600.032.2978672
CGTCGAG900.004089784331.4434261
TACTCGG5700.027.3061313
GCTACTC11350.026.8030951
ACTTGTG9050.021.107054
ACTCGGG7050.021.0737864
AAAGTGC15850.020.9788154
AAGTGCT16200.020.525575
GGAGGCT29700.020.4858689
CCAAAGT18050.019.9896582
CGAGTAG2858.768531E-619.8590053
GACTTGT7900.019.7018953
GCGGGAT1800.004961787719.6521429
TCGGGAG8300.019.6047886
CCCAAAG21100.019.111941
GCTACTT9400.018.8158781
GAGACTT9600.018.4238821
Produced by FastQC (version 0.11.5)