FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences937725
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG4250.049.9493062
TACTCGG5750.041.841593
TAGACCG850.003088278533.2995385
CGGAGGT6600.031.0921827
AAAGTGC16000.029.6313864
GCTACTC11900.029.1370961
GCCGTAC1255.939973E-428.3046071
ACTCGGG7700.027.5694244
TCGGGAG9650.026.3980776
CCAAAGT19900.025.2465722
CTCGGGA8950.024.5095775
AAGTGCT18850.024.0251315
GGAGGCT30000.023.3513019
GGAGGTT16700.021.6098048
CAAAGTG22700.021.1972833
AGGCGGA12300.020.7106884
CCCAAAG23950.020.6818641
TTGGGAG21400.020.5010016
GCTGGGA35300.020.2462129
AGTGCTG23550.019.8312536
Produced by FastQC (version 0.11.5)