FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1029126
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG4150.035.922882
CGGTTAG900.004021970631.5513131
TACTCGG5600.027.8891053
AAAGTGC17900.022.2093054
ACTCGGG7400.021.105274
GCTACTC13150.021.0542031
CGGAGGT6750.021.0342087
AAGTGCT18600.020.9918025
TCGGGAG8900.020.7387836
CCAAAGT20250.019.9824982
GCTTAAT3951.798253E-819.7694931
GGAGGCT34850.019.5554759
CGAGTAG3651.6978083E-719.449443
CTACTTG8800.019.3610322
CTCGGGA9950.019.263745
TCGTAGG1850.005699669519.186612
GCTGGGA34450.017.5157879
GCGTCTT3500.00968247417.500814145
GACTTGT7750.017.4041123
CCCAAAG24600.017.3147451
Produced by FastQC (version 0.11.5)