FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1029126
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGGT7300.027.2344517
GCTACTC13700.026.9504641
AAAGTGC16150.026.8188884
CTACTTG8350.025.5104582
CTACTCG5050.025.3083922
AAGTGCT18350.024.7643765
GTCGACT1751.4466501E-424.3442659
GCTACTT9800.023.1850131
CCGATAC1550.002041653722.904552
TTGGGAG22450.022.771926
CCAAAGT21300.022.6679762
CTACTCA9000.022.0901662
GGAGGCT33750.021.6694019
GCGGAGG10500.021.6393476
CCTATAT3957.6579454E-1021.5708661
CGTAGCT1650.002935457321.5163965
AGGCGGA12000.021.3012314
CCCAAAG23900.020.796181
ACCGGAA2054.2207006E-420.781696
CAAAGTG22850.020.1981033
Produced by FastQC (version 0.11.5)