FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences951096
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGCG400.0064859953.4285744
CTACTCG4700.037.892612
TACTCGG5450.036.5993923
CGGAGGT6100.036.2029727
ACTCGGG7450.026.7740524
GCTACTC11950.025.6337951
AGGCGGA11100.024.387824
CTCGGGA8850.023.343565
GGAGGTT15750.022.615278
CGAGTAG3555.0513336E-922.073783
TCGGGAG8450.021.9194166
AGTAGCT13900.021.0126785
AAAGTGC18000.020.9756644
GCTGGGA36050.020.946579
GGCGGAG12400.020.682035
TTGGGAG21400.020.3061896
GCGGAGG11050.019.9853526
CCCATCG2155.691015E-419.8804022
GGTGGCG6100.019.8532436
GTACCGG1800.004767508219.788367
Produced by FastQC (version 0.11.5)