FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences877634
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCAA6500.027.1760949
GAGACTT8400.024.3937781
GACTTGT7650.024.014433
CTACTTG7300.023.2299772
AGACTTG8300.022.9850832
GTCGAGA1550.00210026222.7928521
CTACTCG3754.2382453E-1022.6105122
CTTGTGA8550.022.3130055
GCTACTT8550.022.3130051
ACTTGTG9350.021.1595694
TTGGGAG19750.021.1079146
GCTAAAC2354.522434E-521.0470161
GTGATCA8950.019.736838
ACTTGGG13250.018.6643354
GCTGGGA30850.018.5519779
GCTACTC9200.018.4324821
GCTATTA4305.0205927E-818.0752641
TACTCGG4406.530172E-817.6644613
TACTTGG10850.017.5830573
AGTAGCT12100.017.5184755
Produced by FastQC (version 0.11.5)