FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2016042
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAATA1308.702045E-943.775592
CTACTCG10500.031.8415322
TACTCGG10950.031.8322473
TATACCG900.003982932331.6157028
CGAGTAG6750.026.3464183
GCCGATA2509.422911E-825.6087191
CGGAGGT13900.024.5647187
CCGATAG1500.001669655923.7117772
TATTCGG2101.8026758E-523.7117753
GCTACTC25400.023.525071
GCTAGTA3403.1250238E-923.0143721
AAAGTGC34350.022.5727844
ACTCGGG15600.022.3437884
TCGGGAG17750.022.0419356
AAGTGCT37200.021.6083135
CCGAGTA8500.020.9221552
CCAAAGT42000.020.1550122
ATTCGGG2909.729105E-619.6235394
AGGCGGA23600.019.5923584
CCCAAAG49800.019.5693591
Produced by FastQC (version 0.11.5)