FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences989470
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG4800.033.052332
GCTACGT1103.2625833E-431.3539871
GTGCGTA1103.2625833E-431.3539874
GCGGGAT1403.8585695E-529.5623329
TACTCGG6100.024.8775923
ATAATCG1802.1304977E-422.9929264
TGCGTAG1550.002415475222.2512195
GCTACTC12750.022.181411
GCACTTT15450.021.8767641
GCTAACC1903.0715857E-421.7827721
TTGGGAG23600.021.6289396
AGTAGCT13950.021.2622745
ACTCGGG7850.021.0890524
GCTACTT9550.020.2241441
CCCAAAG24650.019.3084621
GCTGGGA35500.018.4591089
ACTTTGG17800.018.2134973
CCAAAGT20550.018.125812
AAAGTGC17600.017.6366184
CTACTTG9450.017.518422
Produced by FastQC (version 0.11.5)