FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1079937
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG1753.5852281E-935.830571
CTACTCG3400.034.8352782
GTCGAGA2254.1476596E-827.868222
TACTCGG5200.025.4565473
GACTTGT10700.025.393943
GAGACTT11850.023.5174851
AGACTTG12150.023.5102252
CCCGTAT1500.001883952623.2235181
AAAGTGC17700.023.2235164
CTTGTGA11750.022.5317525
CCAAAGT18250.021.7601172
TGATCAA11650.021.5290959
AAGTGCT19250.020.9916445
GCTACTC10500.020.56941
TTGTGAT13000.020.3652386
GCCGGAT2405.928667E-520.3205761
GTGATCA12550.019.9851788
CGAGACT7850.019.5255031
ACTTGTG14000.019.4082244
CCCAAAG21600.019.352931
Produced by FastQC (version 0.11.5)