FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1079937
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAA1400.001436238438.918575145
TTAGTCG1050.0093352126.5559773
ACTACGG1400.001256606824.896235
CTACTCG4051.8189894E-1224.0970922
GTAACGT1751.6390538E-423.900388
GTACATA2900.001763919323.485346145
TACTCGG4509.094947E-1221.6873823
TGTAACG1953.4096572E-421.4490597
TTGGGAG19800.020.7720056
GCTACTT10200.019.8193511
GCTACTC12200.018.8558311
AACTACG1850.006331981618.840394
TCGGGAG7600.018.344596
AAAGTGC16400.018.2774754
TAACGTT2300.001036507118.1850729
GCTATTA4005.3792064E-717.4273591
CTACTTG8100.017.2122082
CCAACGT4056.126611E-717.2122082
CCAAAGT17400.016.8264162
GGAGGCT33300.016.5376769
Produced by FastQC (version 0.11.5)