FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1107030
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG1302.3413048E-531.8064942
TGATCAA9000.028.3313399
ACTTGTG10850.024.7709564
GAGACTT10100.024.563431
CTACTCG4500.024.502782
ACGTCGA1701.4555077E-424.3226131
GTGATCA11150.024.1044758
GACTTGT10050.023.9999243
TTGTGAT11700.022.3823476
CTTGTGA11300.021.9549265
GTCGAGA2004.371267E-420.6742213
GCTACTC9150.020.33531
TGTGATC13650.020.19467
AGACTTG12550.019.7681792
AAAGTGC16400.019.7497634
CGATTTG1800.00577571919.1427972
CCAAAGT18650.018.475622
GCTAAGT4658.432835E-917.7842751
TACTCGG5953.274181E-1117.3732953
GCTACTT7350.016.8769151
Produced by FastQC (version 0.11.5)