FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1157449
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG600.004293621559.296795145
CTACTCG5950.046.7382242
GCGTAGT750.001609624538.029732
TACTCGG7700.037.041953
GGCGTAT900.00393586631.6914421
CCGCATA1500.00397545831.624954145
GCTACTC16250.030.7163241
TTAACGA1403.077052E-530.5596083
AAAGTGC20800.029.8254
CGAGTAG4150.029.2095853
ACTCGGG10050.029.0899094
TCGGGAG10100.028.9458986
GTTAACG1050.00835963927.1640952
AAGTGCT22400.026.4213285
CCAAAGT24800.026.164612
CTCGGGA11600.025.81765
GGAGGCT37950.024.9898959
TTGGGAG28000.024.1930246
GCTACTT11250.024.0854971
CCGAGTA6000.023.7685832
Produced by FastQC (version 0.11.5)