FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1535713
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG9600.029.371942
TAGTCGC1000.006968400428.1970635
TATATCG1556.661903E-527.2874817
TACTCGG12550.024.1528643
TATTCGG1801.8397355E-423.4975533
ATTGCGG2202.770115E-522.4294824
ACTCGGG13300.022.260844
AGTGCGA3553.2825093E-421.569256145
GACTTGT13450.020.9643573
AAAGTGC32000.020.7072184
CTACTTG15000.020.6778452
AAGTGCT32650.020.510885
GCTACTC22750.020.4506191
TTGGGAG41800.020.405776
TCGGGAG16300.019.8936336
GAATGCG3250.004988628419.633554145
GAGACTT15450.019.6193161
GTCGAGA3251.2875735E-619.5210441
CGCTTGC2207.1340345E-419.2252713
GGAGGCT62950.019.148929
Produced by FastQC (version 0.11.5)