FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1535713
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG10800.028.7211692
CAACGTA1800.00681466428.324759145
CGGAGGT14650.026.9478177
TACTCGG13050.025.9300823
TCGGAAT2550.001228456624.992434145
CGGACCG1500.001759323323.4991389
AGGCGGA22350.022.3951514
CGAGTAG7750.021.8314573
GCTACTC26400.021.6298891
GGCGGAG24850.021.5605755
GGAGGTT32150.021.2698238
ACTCGGG15300.021.1953014
GCACTTT30450.021.0681921
GCGGAGG20500.020.9772786
TCGGGAG18550.020.1421186
GGAGGCG26500.019.4200422
TTGGGAG45100.019.3828816
GGAGGCT63100.019.1046899
CTCGGGA18200.018.9800725
AGTGCGA4058.0456433E-418.883173145
Produced by FastQC (version 0.11.5)