FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences955039
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG4500.032.9543882
CGAGTAG3800.029.7332823
CGACCGG1000.006907627528.2466163
GAATGCG1850.00820955727.262527145
CGTATAC1050.008764408526.901541
TACTCGG5700.026.0166233
AGTAGCT12850.025.8286195
GAGTAGC9700.024.7521924
TTGGGAG20550.024.0542986
CCGAGTA4550.023.2801782
GCTACTC11850.023.2408891
CCGCGCA1650.0030321.3989523
GTAGCTG15700.021.1399866
GCTGGGA34650.020.9913549
CCGCAGT2405.3432505E-520.5964932
CTACTTG8750.020.1761552
GCTACTT10300.019.8823281
ACTCGGG7550.019.641694
TAGCTGG17300.019.5930297
AAAGTGC16950.019.5809884
Produced by FastQC (version 0.11.5)