FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences954693
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG850.00194041436.60858140-144
ATCGCCG850.00301122233.471747
CTACTCG4350.029.4320492
TACTCGG5300.028.1825753
GCCGCTA1050.00846161927.0961741
CCAAAGT18700.021.30022
CAAAGTG20750.020.5669773
GCTACTC11450.020.4996171
AAAGTGC16700.020.443824
TCGGGAG8700.020.4389236
CGAGTAG2455.924255E-520.3221323
GGAGGCT30300.020.1879929
GACTTGT9200.020.1012383
TTGGGAG21150.019.8417026
GATATCG1800.00481058119.7576261
CCGAACT1800.00481058119.7576261
AAGTGCT19350.019.4819375
TGATCAA9150.019.4337319
GACGGGT1850.005636235719.2236377
ACTTGTG9750.018.9673214
Produced by FastQC (version 0.11.5)