FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences954693
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG4950.035.9233672
TACTCGG5700.029.9487463
GCTACTC11750.029.0566541
CGAGTAG3750.026.5545543
CGGAGGT6600.024.7871237
ACTCGGG7550.024.4945034
AAAGTGC16300.023.5639674
AAGTGCT17750.020.8375785
CGCAATG2054.1709223E-420.8180312
AGGCGGA10950.020.7863434
CCAAAGT20250.019.3187892
GCTGGGA32900.019.0251889
GAGTAGC10750.018.5264324
GGAGGCT29800.018.378789
CCGGGTT4706.504706E-918.160418
TAGCTGG16600.017.9963077
CTCGGGA10700.017.9482545
TCGGGAG10450.017.6969836
TTGGGAG19600.017.4191676
AGTAGCT13900.017.3982815
Produced by FastQC (version 0.11.5)