FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences717781
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG4300.036.0265352
TACTCGG5050.034.8591583
GCTACGT1153.7508577E-430.6154371
AAAGTGC12850.025.2071044
ACTCGGG6450.024.0176894
CCAAAGT15650.023.3968432
CCCAAAG17650.023.1393721
AAGTGCT14700.022.9928175
GCTACTC11100.022.2030851
CTACTTG5750.022.0431122
CAAAGTG16650.021.5687143
GTCGAGA1650.00307978621.3380321
TCGGGAG8000.021.124656
CGAGTAG3108.2571205E-720.443213
CGGAGGT5600.020.1187157
GCTGGGA27550.019.6805579
GCTACTT6800.019.674921
AGTAGCT11850.019.6093795
CTCGGGA8300.019.512735
CTACGTG1850.005972092519.0312162
Produced by FastQC (version 0.11.5)