FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences717781
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCCG1000.003398671532.65318140-144
CTACTCG4600.032.1502762
CGCGGGT900.004181845631.299749
CTACTAG1800.00671708128.40484145
GCTCGCG1000.006998983728.1697649
CGGAGGT5350.027.6432297
TACTCGG5950.024.8556773
GCTAACC1751.528756E-424.1455159
GCTACTC11000.023.6882111
ACTCGGG6700.021.0222134
AAGTGCT15450.020.9677895
AAAGTGC14850.020.8664954
AGTAGCT11650.020.553055
TTGGGAG19200.020.5404556
GCGGAGG8500.019.8845426
CGAGTAG3201.1184093E-619.8068663
AGGCGGA10000.019.7188384
CCAAAGT17100.018.944582
CTACTTG6700.018.919992
GCTGGGA28050.018.830069
Produced by FastQC (version 0.11.5)