FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1323499
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA900.004035913431.529675
CGTATAT1204.6095587E-429.5590691
CTACTCG9950.027.8063182
CGGAGGT11500.027.759827
CTACTTG13350.027.6327482
GCTACTC20100.027.5296361
TACTCGG10900.026.6845153
GCTACTT14650.024.2122061
AAAGTGC23800.023.5478954
GACGATT1902.5412577E-422.4026621
GGCGGAG19650.022.383665
TACTTGG17800.022.3187563
GGAGGCT49600.022.312339
GCGGAGG16650.022.1559856
TTGGGAG35200.021.9677626
AGGCGGA19400.021.940754
GCTGGGA51400.021.6689829
AAGTGCT26850.021.6656425
ACTCGGG13150.021.5792434
CCCAAAG32250.021.3375231
Produced by FastQC (version 0.11.5)