FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1188797
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACTT10400.028.224111
GACTTGT11300.025.3720763
TGATCAA11600.024.7159029
ACTTGTG12300.023.3093054
AGACTTG12150.023.035242
CTTGTGA12350.022.66225
TTGTGAT14250.022.5148016
TGTGATC16350.021.2930377
GTGATCA13200.021.2028928
CGAGTAG4652.0008883E-1120.552293
GTCGAGA2004.6606353E-420.478891
CGAGACT10000.020.4788881
AAAGTGC17600.019.0050324
CCAAAGT19450.017.548322
AGTAGCT13400.017.3204545
GCTTACA5300.001501510217.204834145
TAGCTGG16350.017.1179317
CTACTCG6004.3655746E-1117.065742
GTATAGC2400.0015869217.065749
AAGTGCT19250.017.0214165
Produced by FastQC (version 0.11.5)