FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1330952
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA750.00714858552.12914145
CTACTCG6000.030.4652942
CGTCGAG1556.783553E-527.2146511
ACGTCGA1400.001196005125.1087571
TCGGAAT2600.001208505625.062084145
TACGGGT2051.6377999E-524.006425
TACTCGG8050.022.7070483
AAAGTGC22500.022.1849824
GCTACTC16050.020.5876181
TTGGGAG31200.020.280156
AGTAGCT20900.020.1831175
GCTAGCG1750.00437260920.0870061
ATACTCG1800.00514650719.5290364
AAGTGCT26350.019.4771525
GCTGGGA45900.019.2992789
ACTCGGG9300.018.8990634
GCACTTT20450.017.8769441
ACTTGTG14700.017.6956964
CCCAAAG32300.017.6305451
GTCGAGA2801.8050346E-417.576132
Produced by FastQC (version 0.11.5)