FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences807612
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACGT1103.152675E-431.5368771
CGAGTAG2753.007499E-722.7065533
CTACTTG7100.022.4755782
AAGTGCT14500.021.5320765
GCTACTT8250.021.0245841
CCAAAGT15750.020.704212
AAAGTGC13400.020.1930164
CCCAAAG19850.019.5735191
GCTATAT2601.13165355E-418.6795371
TTGGGAG17450.018.2895816
CAAAGTG17550.018.1853683
CATATAG3452.650926E-618.0994263
ACTATAG2400.001422787817.3452845
CCTTTAG4801.1474185E-817.3452841
GCTGGGA30950.017.2612199
GCTACTC9050.016.099491
CTAGGTT3455.358997E-516.0883799
CCTAATA3053.8319128E-415.9235381
GGAGGCT28550.015.5530399
GACTTGT5455.484435E-815.2765793
Produced by FastQC (version 0.11.5)