FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012446
Sequences flagged as poor quality0
Sequence length35-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG6500.037.80672
TACTCGG8300.032.1454543
AAAGTGC20150.027.5274584
ACTCGGG9750.026.6447224
CTCGGGA10700.026.2476435
AAGTGCT21300.026.0412315
TCGGGAG10600.025.8328786
GCTACTT9700.024.6105481
CCAAAGT23500.024.2008842
AGTAGCT17900.023.9271435
GCTACTC15400.023.2521721
GCTGGGA42100.023.0150039
CCCAAAG27300.022.889771
CGGAGGT6850.022.5499827
CAAAGTG24450.021.8247263
CTACTTG9050.021.7231852
CGAGTAG4851.8189894E-1221.7151893
AGGCGGA11500.021.3690034
GAGTAGC12200.021.2939384
TTGGGAG24050.021.019946
Produced by FastQC (version 0.11.5)