FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012446
Sequences flagged as poor quality0
Sequence length35-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG4400.035.1138883
AGTAGCT16400.030.8317075
CCGAGTA5900.028.567232
CCCGAGT7500.027.1547411
CTACTCG7250.027.1224522
GTAGCTG18550.025.7438746
GAGTAGC12050.025.0605344
GCTACTC14250.024.6413251
CGGAGGT9150.024.5605347
CTACTTG9650.023.2879682
AAGTAGC8450.023.2707424
TACTCGG8450.023.2707423
CAAGTAG8200.023.1237813
GCTACTT11250.023.0971361
TAGCTGG21800.022.5502037
GCTGGGA41650.022.4256749
AGGCGGA13200.022.34524
TTGGGAG26800.021.225566
GCACTTT16800.020.4831031
CCAAGTA11450.019.6269762
Produced by FastQC (version 0.11.5)