FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1182858
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG5650.027.8897482
GACGGTT1805.166041E-627.8545349
AAGTGCT28500.026.8911445
CCCAAAG33000.026.6969451
TACGGGT1358.676956E-426.5281285
AAAGTGC26500.026.2177984
GCTGGGA46400.025.4704349
CGATACT2001.17077725E-525.0690823
CCAAAGT29550.023.996512
CGGAGGT9300.023.8753157
TTGGGAG30300.023.8753136
TACTCGG6950.023.7035523
AGTAGCT19700.023.6329255
CAAAGTG30850.022.7531383
GCTACTC15650.021.968341
AGGCGGA14250.021.6134434
AGTGCTG33250.021.3262236
TAGCTGG24900.021.2864257
GTAGCTG23000.020.5535346
GCGGAGG12600.020.4645566
Produced by FastQC (version 0.11.5)