FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1089646
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGA900.00962126448.352764145
CTACTCG4250.030.9368722
GATACGA900.004557308730.7559551
GTCGAGA2102.2657332E-829.6575281
TCGAATA2000.006059899529.01166145
ATACGAG1000.007625783327.6803592
TACTCGG5900.023.4579323
GACTTGT8300.022.5111353
GAGACTT9400.021.3492131
AAAGTGC17500.020.9579874
AAGTGCT18100.020.6455735
ACTTGTG9950.020.1691064
TGATCAA9650.020.0790169
CCAAAGT20650.019.7716872
GCTACTT8800.018.8729721
GTGATCA11150.017.9984428
GCTACTC9650.017.9276941
CTACTTG8500.017.9108222
CCCAAAG24400.017.8674451
CAAAGTG21800.017.7763773
Produced by FastQC (version 0.11.5)